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Image Search Results
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Macrophage-stimulating protein is produced by tubular cells and activates mesangial cells.
doi: 10.1681/ASN.V133649
Figure Lengend Snippet: Figure 1. Western blot performed with anti–macrophage-stimulating protein (MSP) antibody (Ab) in culture supernatant of the human tubular cell line HK2, human mesangial cells (HMC), and the hepa- toma cell line HepG2. A 50 ml volume of supernatant was immuno- precipitated with anti-MSP polyclonal Ab adsorbed to protein A–sepharose 4B packed beads. Immunoprecipitates were washed with an ice-cold buffer, boiled after addition of 2 sample buffer, and the proteins were loaded onto 10% sodium dodecyl sulfate–polyacrylam- ide gel electrophoresis. Proteins were analyzed by Western blot that used a polyclonal anti-MSP Ab. The 85-kD bands represent mono- meric MSP (pro-MSP), and the 55-kD bands represent the -chain of dimeric (active) MSP. Both HK2 and HepG2 cells (used as controls) release pro-MSP that is cleaved into the dimeric form in the super- natant. No band is visible in the HMC lane.
Article Snippet: In some experiments, HMC were incubated with HK2 supernatant or with HK2 supernatant preincubated for 2 h with neutralizing
Techniques: Western Blot, Nucleic Acid Electrophoresis